Development of a HS-SPME method for the analysis of volatile compounds in coffee extracts

Abstract

An analytical method for volatile compounds in coffee hydroalcoholic extracts, based on headspace-solid phase microextracción, was developed and optimized. The optimized conditions were: PDMS/DVB (65 mm) fiber, 2-mL extract at 40% ethanol and 6-mL Milli-Q water, 0.80 g sodium chloride, agitation at 100 rpm, 15 min pre-extraction, 25 min extraction at 50 ^oC and 2 min desorption. The partial validation of the method indicated that it is lineal in the range up to 2 mL of extract, it possesses good repeatability and reproducibility (relative standard deviation lower than 1% for the total area) and it is robust for the evaluated analytical conditions.